rabbit polyclonal anti c jun antibody  (Santa Cruz Biotechnology)

Journal: Journal of neurochemistry

Article Title: Pin1 inhibition activates cyclin D and produces neurodegenerative pathology.

doi: 10.1111/j.1471-4159.2011.07259.x

Figure Lengend Snippet: Fig. 1 Expressional levels of cell cycle-related genes under 3.5–5 lM juglone for 3 h. Untreated cells have a relative expressional value of ‘1’ in each gene that was analyzed. RT-PCR analysis shows a significant increase in cyclin D1 mRNA levels (p = 0.0238, *p < 0.05) but not in cyclin A (p = 0.3996, p > 0.05) or cyclin B1 (p = 0.3803, p > 0.05).

Article Snippet: Membrane was blocked in 3% bovine serum albumin, and probed with primary antibodies [1 : 500; cyclin D1 rabbit polyclonal IgG (Santa Cruz); caspase 3 rabbit polyclonal IgG (Cell Signaling); b-actin polyclonal mouse IgG (Santa Cruz)] in trisbuffered saline–Tween 20 buffer containing 1% bovine serum albumin and 2.5% skimmed milk powder according to manufacturer’s instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction

Journal: Journal of neurochemistry

Article Title: Pin1 inhibition activates cyclin D and produces neurodegenerative pathology.

doi: 10.1111/j.1471-4159.2011.07259.x

Figure Lengend Snippet: Fig. 2 Cyclin D1 expression and morpho- logical changes such as axonal retraction in 3.5 lM juglone for 2.5 h and 4 lM juglone for 3 h of incubation, b-III tubulin (green), cyclin D1 (red), DAPI (blue) (a) and inte- grated pixel analysis for cyclin D1 expres- sions (***p < 0.0001, considered extremely significant) (b).

Article Snippet: Membrane was blocked in 3% bovine serum albumin, and probed with primary antibodies [1 : 500; cyclin D1 rabbit polyclonal IgG (Santa Cruz); caspase 3 rabbit polyclonal IgG (Cell Signaling); b-actin polyclonal mouse IgG (Santa Cruz)] in trisbuffered saline–Tween 20 buffer containing 1% bovine serum albumin and 2.5% skimmed milk powder according to manufacturer’s instructions.

Techniques: Expressing, Incubation

Journal: Journal of neurochemistry

Article Title: Pin1 inhibition activates cyclin D and produces neurodegenerative pathology.

doi: 10.1111/j.1471-4159.2011.07259.x

Figure Lengend Snippet: Fig. 3 Immunohistochemistry analysis for cyclin D1 expression in rat brains after receiving 0.1 and 1 mg/kg juglone for 3 weeks. An increase in expression of cy- clin D1 can be observed in hippocampus, thalamus, and cortex.

Article Snippet: Membrane was blocked in 3% bovine serum albumin, and probed with primary antibodies [1 : 500; cyclin D1 rabbit polyclonal IgG (Santa Cruz); caspase 3 rabbit polyclonal IgG (Cell Signaling); b-actin polyclonal mouse IgG (Santa Cruz)] in trisbuffered saline–Tween 20 buffer containing 1% bovine serum albumin and 2.5% skimmed milk powder according to manufacturer’s instructions.

Techniques: Immunohistochemistry, Expressing

Journal: Journal of neurochemistry

Article Title: Pin1 inhibition activates cyclin D and produces neurodegenerative pathology.

doi: 10.1111/j.1471-4159.2011.07259.x

Figure Lengend Snippet: Fig. 4 Cyclin A expression in untreated cells, at 4 lM juglone for 3 h of incubation and non-neuronal cells, b-III tubulin (green), cyclin A (red), DAPI (blue), axonal retraction, and a condensed nuclear localization for cyclin A is visible (a) and integrated pixel analysis for cyclin A expression shows that there is no significant change after juglone treatment (p = 0.7342, considered not significant) (b).

Article Snippet: Membrane was blocked in 3% bovine serum albumin, and probed with primary antibodies [1 : 500; cyclin D1 rabbit polyclonal IgG (Santa Cruz); caspase 3 rabbit polyclonal IgG (Cell Signaling); b-actin polyclonal mouse IgG (Santa Cruz)] in trisbuffered saline–Tween 20 buffer containing 1% bovine serum albumin and 2.5% skimmed milk powder according to manufacturer’s instructions.

Techniques: Expressing, Incubation

Journal: Journal of neurochemistry

Article Title: Pin1 inhibition activates cyclin D and produces neurodegenerative pathology.

doi: 10.1111/j.1471-4159.2011.07259.x

Figure Lengend Snippet: Fig. 6 Western blotting analysis shows increase in cyclin D1 and caspase 3 protein levels (caspase 3 antibody detects the pro-caspase form at p32 and the cleaved, active form at p17) after Pin1 inhibition by 4 lM juglone for 6 h in post-natal 0 (PN0) rat hippocampal neurons (a). Integrated pixel analysis for western blotting shows the relative intensity to the untreated protein level of ‘1’ for the observed proteins. There is over twofold change for cyclin D1, 3.5-fold increase in pro- caspase 3, and 1.5-fold increase in active caspase 3 (b).

Article Snippet: Membrane was blocked in 3% bovine serum albumin, and probed with primary antibodies [1 : 500; cyclin D1 rabbit polyclonal IgG (Santa Cruz); caspase 3 rabbit polyclonal IgG (Cell Signaling); b-actin polyclonal mouse IgG (Santa Cruz)] in trisbuffered saline–Tween 20 buffer containing 1% bovine serum albumin and 2.5% skimmed milk powder according to manufacturer’s instructions.

Techniques: Western Blot, Inhibition